Langerin (CD207) Mouse Monoclonal Antibody [Clone ID: 808E10.01]

Specifications

Product Data
Clone Name	808E10.01
Applications	FC, FN, IHC
Recommend Dilution	Flow cytometry, blocks HIVgp120, IHC. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Langerin-transfected eukaryotic cells
Specificity	Human Langerin (extracellular epitope). Species cross-reactivity: Mouse.
Isotype	IgG1
Formulation	Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	CD207 molecule
Database Link	Entrez Gene 50489 Human UniProt ID Q9UJ71
Background	Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 2002; J. Immunol., 168 : 782-779 ; Douillard P et al, 200; J. Invest. Dermatol., 125: 983–994)
Synonyms	CLEC4K, Dendritic Cell Marker
Note	Protocol: Langerin-gp120 blocking protocol: Mock- and Langerin-transfected CHO cells are incubated with biotin-labelled gp160 (5μg/ml) and clone 808E10.01 at (1 to 10μg/ml) in PBS 45 min. at RT. Cells were washed in PBS 5min at 1600rpm, and stained with Streptavidin-PE 30 min at 4°C (DAKO). A last PBS wash is performed before Flow cytometry analysis.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.