Interleukin-4 / IL4 Rat Monoclonal Antibody [Clone ID: 11B4]

Specifications

Product Data
Clone Name	11B4
Applications	ELISA, IP, Neutralize
Recommend Dilution	ELISA, IP, Neutralization of IL4 bioactivity.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human IL4-transfected Cos7 cells.
Specificity	Human interleukin-4.
Isotype	IgG2a
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. State: Purified
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Database Link	NP_000580.1 Entrez Gene 3565 Human
Background	Interleukin-4 (IL-4) is a monomeric 20 kDa predominantly produced by activated T lymphocytes of the Th2 phenotype, by mast cells and by some antigen-presenting cells. Together with IL-2, IL-4 is an autocrine growth factor for activated T cells and for cytotoxic T cell precursors. IL-4 is a major factor involved in the activation of resting B lymphocytes. Together with IL-13, IL-4 induces isotype switch towards IgE and IgG1 isotypes. 11B4, was produced from a rat immunized with purified human IL4-transfected-COS7 cells. It recognized both E. coli-expressed and mammalian cell-expressed (COS7 and L cell), rhuIL-4 in solution (immunoprecipitation), as well as on solid phase (indirect ELISA and dot-blotting). The 11B4 antibody inhibited IL-4 bioactivity at an IC50 which was 25-50-fold in molar excess of factor. The 11B4 antibody was used to develop an immunoenzymatic assay capable of detecting concentrations less than 100 pg/ml. (Chrétien I et al, 1989, J. Immunol. Methods, 1178 (1) : 67-81; Ramanathan L.et al, 1993, Biochemistry, 32 (14) :3549-56).
Synonyms	IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway

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