LAMP3 Mouse Monoclonal Antibody [Clone ID: 208B5]

Specifications

Product Data
Clone Name	208B5
Applications	FC
Recommend Dilution	DDX0195P-50 / DDX0195P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections. DDX0195A488-50 / DDX0195A488-100 Alexa-fluor®488: Intra Flow cytometry for mouse and human. DDX0195A546-50 / DDX0195A546-100 Alexa-fluor®546: FACS intracellular, ImmunoHistoChemistry frozen sections. DDX0195A647-50 / DDX0195A647-100 Alexa-fluor®647: Intra Flow cytometry for mouse and human. DDX0195B-50 / DDX0195B-100 Biotin: FACS intracellular, ImmunoHistoChemistry frozen sections. Usage recommendation: *This monoclonal antibody may be used between 5-10 μg/ml.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Murine DC-LAMP
Specificity	Murine DC-LAMP (epitope in intracytoplasmic domain). Species cross-reactivity: Human.
Isotype	IgG3
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D Ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	lysosomal associated membrane protein 3
Database Link	Entrez Gene 27074 Human UniProt ID Q9UQV4
Background	The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in the mouse and 90 kDa in human (due to extensive glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. DC-LAMP is specifically expressed by human DCs upon activation. However, its mouse counterpart has not be detected in mature DCs. DC-LAMP is constitutively expressed by mouse. Confocal and immunoelectron microscopy has shown that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contains lung surfactant protein B (Salaun B et al, 2004; Eur. J. Immunol., 33: 2619-29).
Synonyms	DCLAMP, DC-LAMP, TSC403, Dendritic Cell Marker
Reference Data

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