Ly6g Rat Monoclonal Antibody [Clone ID: RB6-8C5]

Specifications

Product Data
Clone Name	RB6-8C5
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections. Western Blotting. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/100-1/200 to label 106 cells in 100 µl.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Normal murine bone marrow cells.
Specificity	This antibody recognizes the Mouse Gr-1 antigen. Clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated. This Clone RB6-8C5 has been reported to deplete mouse mature neutrophils in vivo (3). We recommend the use of CL046LE for functional studies.
Isotype	IgG2b
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified Ig fraction from tissue culture supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lymphocyte antigen 6 complex, locus G
Database Link	Entrez Gene 546644 Mouse UniProt ID P35461
Background	The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported.
Synonyms	Gr-1 Granulocyte marker
Reference Data

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