CD11a / ITGAL Mouse Monoclonal Antibody [Clone ID: 8-6.2]

Specifications

Product Data
Clone Name	8-6.2
Applications	FC
Recommend Dilution	Flow Cytometry (See Protocols).
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	B6-Ly-1a Thymus, spleen and lymph node. Donor: 129/ReJ spleen Fusion Partner: P3-NS-1 Ag-4
Specificity	This CD11a Monoclonal Antibody identifies a cell surface glycoprotein consisting of two non-covalently associated chains with molecular weights of 180kDa (Alpha chain) (1) present on most common lymphocytes and T and B cells.
Isotype	IgG2a
Formulation	PBS containing 0.02% Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in alqiuots) -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P24063
Background	CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.
Synonyms	Integrin alpha-L, LFA1, LFA-1
Note	Protocol: Flow Cytometry Analysis: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x106 cells, representing 1 test). 4. To each tube, add 0.2-0.5 µg* of CL013P. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of FITC Goat anti-Mouse IgG (H+L)) secondary antibody at a 1:500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: C57BL/6 Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 0.5 µg/106 cells Isotypic Control: Purified Mouse IgG2a Cell Source Percentage of cells stained above control: Thymus: 99.7% Strain Distibution: Antibody Concentration Used: 1/2000 Strains Tested: C57BL/6, BALB/c, AKR, CBA/J, C3H/HE Positive: C57BL/6, CBA/J, C3H/He Negative: BALB/c, AKR
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.