RPE65 Mouse Monoclonal Antibody [Clone ID: OTI6D6]

Specifications

Product Data
Clone Name	OTI6D6
Applications	WB
Recommend Dilution	WB 1:500-1:5000
Reactivity	Human,Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment of human RPE65 (NP_000320) produced in E.coli.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	61.4 kDa
Gene Name	retinoid isomerohydrolase RPE65
Database Link	NP_000320 Entrez Gene 19892 MouseEntrez Gene 6121 Human UniProt ID Q16518
Background	The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017]
Synonyms	BCO3; LCA2; mRPE65; p63; rd12; RP20; sRPE65
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Retinol metabolism

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