EEA1 Mouse Monoclonal Antibody [Clone ID: OTI2F6]

Specifications

Product Data
Clone Name	OTI2F6
Applications	WB
Recommend Dilution	WB 1:500-1000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-400 of human EEA1 (NP_003557) produced in E.coli.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	162.3 kDa
Gene Name	early endosome antigen 1
Database Link	NP_003557 Entrez Gene 216238 MouseEntrez Gene 314764 RatEntrez Gene 8411 Human UniProt ID Q15075
Background	Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. [UniProtKB/Swiss-Prot Function]
Synonyms	MST105; MSTP105; ZFYVE2
Reference Data
Protein Pathways	Endocytosis

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.