PSMA1 Mouse Monoclonal Antibody [Clone ID: OTI9H1]

Specifications

Product Data
Clone Name	OTI9H1
Applications	IF, WB
Recommend Dilution	WB 1:250, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PSMA1 (NP_002777) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	29.4 kDa
Gene Name	proteasome 20S subunit alpha 1
Database Link	NP_002777 Entrez Gene 26440 MouseEntrez Gene 29668 RatEntrez Gene 5682 Human UniProt ID P25786
Background	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2009]
Synonyms	HC2; HEL-S-275; NU; PROS30
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Proteasome

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