METTL3 Mouse Monoclonal Antibody [Clone ID: OTI1B7]

Specifications

Product Data
Clone Name	OTI1B7
Applications	Simple Western, WB
Recommend Dilution	WB 1:500~2000, Simple Western 1:20-1:50
Reactivity	Human, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-284 of human METTL3 (NP_062826) produced in E.coli.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	64.3 kDa
Gene Name	methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit
Database Link	NP_062826 Entrez Gene 56335 MouseEntrez Gene 361035 RatEntrez Gene 56339 Human UniProt ID Q86U44
Background	This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
Synonyms	IME4; M6A; MT-A70; Spo8
Reference Data

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