ALAS1 Mouse Monoclonal Antibody [Clone ID: OTI5D5]

Specifications

Product Data
Clone Name	OTI5D5
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 57-308 of human ALAS1(NP_000679) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	70.4 kDa
Gene Name	5'-aminolevulinate synthase 1
Database Link	NP_000679 Entrez Gene 11655 MouseEntrez Gene 65155 RatEntrez Gene 211 Human UniProt ID P13196
Background	This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]
Synonyms	ALAS; ALAS-H; ALAS3; ALASH; MIG4
Reference Data
Protein Pathways	Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism

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