ErbB 4 (ERBB4) Mouse Monoclonal Antibody [Clone ID: OTI1H6]

Specifications

Product Data
Clone Name	OTI1H6
Applications	IHC, WB
Recommend Dilution	IHC 1:1000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 992-1308 of human ERBB4 (NP_005226) produced in E.coli.
Isotype	IgG2b
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	144.2 kDa
Gene Name	erb-b2 receptor tyrosine kinase 4
Database Link	NP_005226 Entrez Gene 13869 MouseEntrez Gene 59323 RatEntrez Gene 2066 Human UniProt ID Q15303
Background	This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Synonyms	ALS19; HER4; p180erbB4
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Calcium signaling pathway, Endocytosis, ErbB signaling pathway

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