EZH2 Mouse Monoclonal Antibody [Clone ID: OTI10B10]

Specifications

Product Data
Clone Name	OTI10B10
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 182-431 of human EZH2 ( NP_004447) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	85.8 kDa
Gene Name	enhancer of zeste 2 polycomb repressive complex 2 subunit
Database Link	NP_004447 Entrez Gene 14056 MouseEntrez Gene 312299 RatEntrez Gene 2146 Human UniProt ID Q15910
Background	This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
Synonyms	ENX-1; ENX1; EZH1; EZH2b; KMT6; KMT6A; WVS; WVS2
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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