DOT1L Mouse Monoclonal Antibody [Clone ID: OTI8A11]

Specifications

Product Data
Clone Name	OTI8A11
Applications	WB
Recommend Dilution	WB 1:2000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1008-1424 of human DOT1L (NP_115871) produced in E.coli.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	164.7 kDa
Gene Name	DOT1 like histone lysine methyltransferase
Database Link	NP_115871 Entrez Gene 84444 Human UniProt ID Q8TEK3
Background	The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
Synonyms	DOT1; KMT4
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Lysine degradation

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