GAPDH Mouse Monoclonal Antibody [Clone ID: OTI2D9]

Specifications

Product Data
Clone Name	OTI2D9
Applications	Simple Western, WB
Recommend Dilution	WB 1:500000 @1mg/ml (Hypersensitive substrate ECL) ; Simple Western 1:20-1:50
Reactivity	Human, Mouse, Rat, Dog, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GAPDH (NP_002037) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35.9 kDa
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	NP_002037 Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 403755 DogEntrez Gene 574353 MonkeyEntrez Gene 2597 Human UniProt ID P04406
Background	The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Synonyms	G3PD; GAPD; HEL-S-162eP
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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