Neurofilament (NEFM) Mouse Monoclonal Antibody [Clone ID: OTI1A4]

Specifications

Product Data
Clone Name	OTI1A4
Applications	IF, WB
Recommend Dilution	WB 1:1000 -1:4000, IF 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human NEFM(NP_005373) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	102.3 kDa
Gene Name	neurofilament medium chain
Database Link	NP_005373 Entrez Gene 4741 Human UniProt ID P07197
Background	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Synonyms	NEF3; NF-M; NFM
Reference Data
Protein Pathways	Amyotrophic lateral sclerosis (ALS)

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