Chromogranin A (CHGA) Mouse Monoclonal Antibody [Clone ID: OTI2F2]

Specifications

Product Data
Clone Name	OTI2F2
Applications	IF, IHC, Simple Western, WB
Recommend Dilution	WB 1:4000, IHC 1:500, IF 1:100, Simple Western 1:20-1:50
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human CHGA(NP_001266) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	48.9 kDa
Gene Name	chromogranin A
Database Link	NP_001266 Entrez Gene 1113 Human UniProt ID P10645
Background	The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008]
Synonyms	CGA
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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