RHOJ Mouse Monoclonal Antibody [Clone ID: OTI1D7]

Specifications

Product Data
Clone Name	OTI1D7
Applications	IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	23.6 kDa
Gene Name	ras homolog family member J
Database Link	NP_065714 Entrez Gene 80837 MouseEntrez Gene 299145 RatEntrez Gene 57381 Human UniProt ID Q9H4E5
Background	ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis. [supplied by OMIM]
Synonyms	ARHJ; RASL7B; TC10B; TCL
Reference Data
Protein Families	Druggable Genome

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