DBT Mouse Monoclonal Antibody [Clone ID: OTI1G2]

Specifications

Product Data
Clone Name	OTI1G2
Applications	FC, IF, WB
Recommend Dilution	WB 1:2000, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	46.4 kDa
Gene Name	dihydrolipoamide branched chain transacylase E2
Database Link	NP_001909 Entrez Gene 13171 MouseEntrez Gene 29611 RatEntrez Gene 1629 Human UniProt ID P11182
Background	The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]
Synonyms	BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Valine, leucine and isoleucine degradation

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