Apg3 (ATG3) Mouse Monoclonal Antibody [Clone ID: OTI3H2]

Specifications

Product Data
Clone Name	OTI3H2
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35.7 kDa
Gene Name	autophagy related 3
Database Link	NP_071933 Entrez Gene 67841 MouseEntrez Gene 171415 RatEntrez Gene 478564 DogEntrez Gene 708305 MonkeyEntrez Gene 64422 Human UniProt ID Q9NT62
Background	Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM, Mar 2008]
Synonyms	APG3; APG3-LIKE; APG3L; PC3-96
Reference Data
Protein Pathways	Regulation of autophagy

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