PDE1B Mouse Monoclonal Antibody [Clone ID: OTI6E4]

Specifications

Product Data
Clone Name	OTI6E4
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
Isotype	IgG2b
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	61.2 kDa
Gene Name	phosphodiesterase 1B
Database Link	NP_000915 Entrez Gene 18574 MouseEntrez Gene 29691 RatEntrez Gene 5153 Human UniProt ID Q01064
Background	Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]). [supplied by OMIM]
Synonyms	PDE1B1; PDES1B
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism

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