AP2M1 Mouse Monoclonal Antibody [Clone ID: OTI 2F11]

Specifications

Product Data
Clone Name	OTI 2F11
Applications	FC, IHC, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, FLOW 1:100
Reactivity	Human, Dog, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1 (NP_001020376) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	49.2 kDa
Gene Name	adaptor related protein complex 2 subunit mu 1
Database Link	NP_001020376 Entrez Gene 11773 MouseEntrez Gene 116563 RatEntrez Gene 607139 DogEntrez Gene 1173 Human UniProt ID Q96CW1
Background	This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Synonyms	AP50; CLAPM1; mu2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Endocytosis, Huntington's disease

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