eRF1 (ETF1) Mouse Monoclonal Antibody [Clone ID: OTI2E3]

Specifications

Product Data
Clone Name	OTI2E3
Applications	FC, WB
Recommend Dilution	WB 1:500, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	48.9 kDa
Gene Name	eukaryotic translation termination factor 1
Database Link	NP_004721 Entrez Gene 307503 RatEntrez Gene 2107 Human UniProt ID P62495
Background	This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012]
Synonyms	D5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1
Reference Data

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