FADD Mouse Monoclonal Antibody [Clone ID: OTI1C11]

Specifications

Product Data
Clone Name	OTI1C11
Applications	FC, WB
Recommend Dilution	WB 1:2000, FLOW 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human FADD (NP_003815) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	23.1 kDa
Gene Name	Fas associated via death domain
Database Link	NP_003815 Entrez Gene 8772 Human UniProt ID Q13158
Background	The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq]
Synonyms	GIG3; MORT1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Apoptosis, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway

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