PIM2 Mouse Monoclonal Antibody [Clone ID: OTI5D5]

Specifications

Product Data
Clone Name	OTI5D5
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	34 kDa
Gene Name	Pim-2 proto-oncogene, serine/threonine kinase
Database Link	NP_006866 Entrez Gene 18715 MouseEntrez Gene 317366 RatEntrez Gene 11040 Human UniProt ID Q9P1W9
Background	This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia

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