Kindlin 2 (FERMT2) Mouse Monoclonal Antibody [Clone ID: OTI25A4]

Specifications

Product Data
Clone Name	OTI25A4
Applications	IF, IHC, IP, WB
Recommend Dilution	WB 1:1000~2000, IHC 1:150, IF 1:100, IP 2ug/500ul
Reactivity	Human, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	77.9 kDa
Gene Name	FERM domain containing kindlin 2
Database Link	NP_006823 Entrez Gene 218952 MouseEntrez Gene 289992 RatEntrez Gene 693958 MonkeyEntrez Gene 10979 Human UniProt ID Q96AC1
Background	Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites
Synonyms	KIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B
Reference Data

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