Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI10E5]

Specifications

Product Data
Clone Name	OTI10E5
Applications	IF, IHC, WB
Recommend Dilution	WB 1:1000 IHC 1:50 IF 1:100 Flow 1:500
Reactivity	Human, Dog, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human SILV expression vector
Isotype	IgG2b
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	70.3 kDa
Gene Name	premelanosome protein
Database Link	NP_008859 Entrez Gene 362818 RatEntrez Gene 481102 DogEntrez Gene 710921 MonkeyEntrez Gene 6490 Human UniProt ID P40967
Background	Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity
Synonyms	D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV
Reference Data
Protein Families	Secreted Protein, Transmembrane

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