ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI3F12]

CAT#: CF500063

Carrier-free (BSA/glycerol-free) DFF45 mouse monoclonal antibody, clone OTI3F12 (formerly 3F12)

Formulation: Standard Carrier-Free



  View other "OTI3F12" antibodies (4)

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CNY 3,999.00


货期*
2周

规格
    • 100 ug

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Specifications

Product Data
Clone Name OTI3F12
Applications IF, WB
Recommend Dilution WB 1:200~500, IF 1:100
Reactivity Human, Monkey
Host Mouse
Clonality Monoclonal
Immunogen Full length human recombinant protein of human DFFA (NP_004392) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 36.3 kDa
Gene Name DNA fragmentation factor subunit alpha
Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Synonyms DFF-45; DFF1; ICAD
Reference Data
Protein Pathways Apoptosis
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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