Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI1B12]

Specifications

Product Data
Clone Name	OTI1B12
Applications	IF, IHC, Simple Western, WB
Recommend Dilution	WB 1:2500~5000, IHC 1:50 - 1:150, IF 1:50 - 1:100, Simple Western 1:20-1:50
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
Isotype	IgG2b
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	53.5 kDa
Gene Name	keratin 8
Database Link	NP_002264 Entrez Gene 16691 MouseEntrez Gene 25626 RatEntrez Gene 486513 DogEntrez Gene 3856 Human UniProt ID P05787
Background	Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Synonyms	CARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Reference Data
Protein Families	Druggable Genome

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