Collagen VI (COL6A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	ELISA: 1/200 (OD ≥ 500). RIA.  Immunofluorescence: 1/80. Immunohistology on Frozen and Paraffin Embedded Sections. For paraffin: 1/400-1/1000, 60 min at RT or 2-8°C over night for human collagen type VI, lower dilution is recommended for other species). Positive Control: Human placenta. Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Reactivity	Bovine, Chicken, Human, Mouse, Porcine, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified collagen type VI from human placenta.
Specificity	Human Collagen type VI: 100% binding in RIA at 1/10,000 dilution. Cross-reactivity: Human Collagen type I, II, III, IV and V: < 0.1%.  Human Elastin: < 0.1%. Human Laminin: < 0.1%. Human Fibronectin: < 1%.
Formulation	PBS containing no BSA or preservative. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 0.1 distilled water. For further dilution use appropriate antibody diluent.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	collagen type VI alpha 1
Database Link	Entrez Gene 12833 MouseEntrez Gene 294337 RatEntrez Gene 1291 Human UniProt ID P12109
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea.
Synonyms	COL6A1
Reference Data
Protein Pathways	ECM-receptor interaction, Focal adhesion

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