Fn1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, R
Recommend Dilution	This antibody can be used on Frozen and Paraffin Embedded Tissues: IFA (1/80), IHC(P) (1/500) After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation time: IHC(P) 60 min at RT or 2-8°C over night. Also suitable for ELISA (1/200) and RIA.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified fibronectin from Murine plasma.
Specificity	Murine Fibronectin 100% Murine Collagen type I, III and IV all < 0.1% Murine Laminin < 0.1% (RIA at 1/10000 dilution).
Formulation	PBS without preservative or stabilizer. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water, dilute further with appropriate antibody diluent.
Concentration	~1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Sore the antibody undiluted 2-8°C for one month or (in aliquots) at -20°C longer. Avoid repeated freezing and thawing.
Gene Name	fibronectin 1
Database Link	Entrez Gene 14268 Mouse UniProt ID P11276
Background	Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
Synonyms	FN1, Cold-insoluble globulin, CIG
Reference Data

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