Collagen I (COL1A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	ELISA: > 1/200. RIA. Immunofluorescence: 1/80. Immunohistochemistry on Paraffin Sections: 1/200-1/600 for 60 min at RT or 2-8°C over night. Positive Control: Tuna Fish skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Reactivity	Bonito, Fish, Tuna
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Collagen type I from Tuna Fish skin
Specificity	This antibody is specific for Fish Collagen type I (Tuna Fish).
Formulation	Phosphate Buffered solution State: Purified State: Lyophilized purified Ig fraction Stabilizer: None Preservative: None
Reconstitution Method	Restore with 0.1 ml distilled water, for further dilution use appropriate antibody diluent
Concentration	~1 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen type I alpha 1
Database Link	Entrez Gene 1277 Human UniProt ID P02452
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion

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