Collagen IV (COL4A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot (Immunoblotting). ELISA (Reacts exclusively with Collagen IV). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Proteolytic treatment required (Enzyme: Pepsin). Recommended Positive Control: Skin, Kidney. Working Dilution: 1/50-1/100. Incubation Time: 1 h at RT or overnight at 2-8°C.
Reactivity	Canine, Feline, Fish, Human, Mouse, Porcine, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Human placental type IV Collagen.
Specificity	The antibody is reactive with Collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. In Immunohistochemistry no cross-reactivity with other Collagens at optimal dilutions. In Immunoblotting, a slight cross-reactivity with Collagen type V is observed.
Formulation	Liquid stabilized antiserum with 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen type IV alpha 1 chain
Database Link	Entrez Gene 12826 MouseEntrez Gene 290905 RatEntrez Gene 1282 Human UniProt ID P02462
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).
Synonyms	COL4A1
Reference Data
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer

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