Nephrin (NPHS1) (1243-1256) Guinea Pig Polyclonal Antibody
CAT#: BP5030
Nephrin (NPHS1) (1243-1256) guinea pig polyclonal antibody, Serum
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CNY 5,929.00
货期*
5周
规格
Cited in 18 publications. |
Specifications
Product Data | |
Applications | IF, IHC, WB |
Recommend Dilution | Western blot: 1/500. Immunohistochemistry/Immunofluorescence Microscopy on frozen tissue: 1/50. Immunohistochemistry on Paraffin Sections: 1/50 (microwave treatment recommended). Incubation Time: 1 h at RT or overnight at 2-8°C. |
Reactivity | Human, Mouse |
Host | Guinea Pig |
Clonality | Polyclonal |
Immunogen | Synthetic peptide (intracellular domain, aa1243-1256) EPGSLPFELRGHLV |
Specificity | The antibody reacts specifically with Nephrin. Immunolocalization: Antibody BP5030 stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas. Tested Reactivities on Cultured Cell Lines: PCL (Podocyte Cell Line), M-1 (Cortical Collecting Duct Cells). |
Formulation | State: Serum State: Whole Antiserum Preservative: 0.09% Sodium Azide |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | NPHS1 nephrin |
Database Link | |
Background | Nephrin was first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm. Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. |
Synonyms | NPHS1, NPHN |
Reference Data |
Citations (18)
The use of this Antibodies has been cited in the following citations: |
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Soluble Klotho protects against glomerular injury through regulation of ER stress response
,null,
Communications Biology
,PubMed ID 36813870
[NPHS1]
|
The classical pathway triggers pathogenic complement activation in membranous nephropathy.
,null,
Nature communications
,PubMed ID 36709213
[NPHS1]
|
Rap1 Activity Is Essential for Focal Adhesion and Slit Diaphragm Integrity
,null,
Frontiers in Cell and Developmental Biology
,PubMed ID 35372328
[NPHS1]
|
GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening
,null,
Communications Biology
,PubMed ID 34857869
[NPHS1]
|
SHROOM3, the gene associated with chronic kidney disease, affects the podocyte structure
,Matsuura, R;Hiraishi, A;Holzman, LB;Hanayama, H;Harano, K;Nakamura, E;Hamasaki, Y;Doi, K;Nangaku, M;Noiri, E;,
Scientific reports
,PubMed ID 33273487
[NPHS1]
|
RAB-RABPHILIN SYSTEM IN INJURED HUMAN PODOCYTES STRESSED BY GLUCOSE OVERLOAD AND ANGIOTENSIN II
,Martinez-Arroyo, O;Ortega, A;Perez-Hernandez, J;Chaves, FJ;Redon, J;Cortes, R;,
Am. J. Physiol. Renal Physiol.
,PubMed ID 32567349
[NPHS1]
|
Xenon blunts NF-κB/NLRP3 inflammasome activation and improves acute onset of accelerated and severe lupus nephritis in mice.
,Yang, S;Hua, K;Chu, L;Hwu, Y;Yang, S;Wu, C;Lin, T;Weng, J;Zhao, H;Hsu, W;Liu, F;Liaw, W;Ma, D;Ka, S;Chen, A;,
Kidney International
[NPHS1]
|
Towards an in vitro model of glomerular barrier unit with an innovative bioassembly method
,Flegeau, K;Rubin, S;Mucha, S;Bur, P;Préterre, J;Siadous, R;L'Azou, B;Fricain, JC;Combe, C;Devillard, R;Kalisky, J;Rigothier, C;,
Nephrol. Dial. Transplant.
,PubMed ID 31121032
[NPHS1]
|
On the Ultrastructure and Function of Rhogocytes from the Pond Snail Lymnaea stagnalis
,Kokkinopoulou M, Spiecker L, Messerschmidt C, Barbeck M, Ghanaati S, Landfester K, Markl J,
PLoS ONE
,PubMed ID 26488403
[NPHS1]
|
The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation
,Yates LL, Papakrivopoulou J, Long DA, Goggolidou P, Connolly JO, Woolf AS, Dean CH,
Hum. Mol. Genet.
,PubMed ID 20843830
[NPHS1]
|
Proteinuria in mice expressing PKB/SGK-resistant GSK3
,Boini KM, Amann K, Kempe D, Alessi DR, Lang F,
Am. J. Physiol. Renal Physiol.
,PubMed ID 18987114
[NPHS1]
|
Systems biology analysis reveals role of MDM2 in diabetic nephropathy
,Saito R, Rocanin-Arjo A, You YH, Darshi M, Van Espen B, Miyamoto S, Pham J, Pu M, Romoli S, Natarajan L, Ju W, Kretzler M, Nelson R, Ono K, Thomasova D, Mulay SR, Ideker T, D'Agati V, Beyret E, Belmonte JC, Anders HJ, Sharma K,
JCI Insight
,PubMed ID 27777973
[NPHS1]
|
Murine Double Minute-2 Inhibition Ameliorates Established Crescentic Glomerulonephritis
,Mulay SR, Romoli S, Desai J, Honarpisheh MM, Kumar SV, Anders HJ, Thomasova D,
Am. J. Pathol.
,PubMed ID 27102769
[NPHS1]
|
Generating conditionally immortalised podocyte cell lines from wild-type mice.
,null,
Nephron
,PubMed ID 25720381
[NPHS1]
|
Murine Double Minute-2 Prevents p53-Overactivation-Related Cell Death (Podoptosis) of Podocytes
,Thomasova D, Bruns HA, Kretschmer V, Ebrahim M, Romoli S, Liapis H, Kotb AM, Endlich N, Anders HJ,
J. Am. Soc. Nephrol.
,PubMed ID 25349197
[NPHS1]
|
Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney
,Okamoto K, Honda K, Doi K, Ishizu T, Katagiri D, Wada T, Tomita K, Ohtake T, Kaneko T, Kobayashi S, Nangaku M, Tokunaga K, Noiri E,
Am. J. Pathol.
,PubMed ID 25987249
[NPHS1]
|
Marker expression, behaviors, and responses vary in different lines of conditionally immortalized cultured podocytes
,Chittiprol S, Chen P, Petrovic-Djergovic D, Eichler T, Ransom RF,
Am. J. Physiol. Renal Physiol.
,PubMed ID 21632959
[NPHS1]
|
Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome
,Huber TB, Hartleben B, Winkelmann K, Schneider L, Becker JU, Leitges M, Walz G, Haller H, Schiffer M,
J. Am. Soc. Nephrol.
,PubMed ID 19279126
[NPHS1]
|
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