Helicobacter pylori Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC
Recommend Dilution	Immunohistochemistry (formalin-fixed paraffin embedded). ELISA (> 1/5,000). Immunofluorescence microscopy (> 1/1,000). Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC.
Reactivity	Helicobacter pylori
Host	Rabbit
Clonality	Polyclonal
Immunogen	ATTC strain 43504.
Specificity	The antibody reacts with whole cell Lysate. This antiserum has not been absorbed and may react with related microorganisms.
Formulation	0.01 M PBS, pH 7.2, containing 10 mg/ml BSA as stabilizer and 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified Ig fraction. Label: High purity Isomer I of Fluorescein Isothiocyanate Care is taken to ensure complete removal of any free fluorescein from the final product
Concentration	lot specific
Purification	Protein A affinity chromatography.
Conjugation	FITC
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Protect from light!
Background	The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources.
Synonyms	H. pylori
Reference Data

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