beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: B2M-01]

Specifications

Product Data
Clone Name	B2M-01
Applications	ELISA, FC, IF, IHC, IP, R, WB
Recommend Dilution	Western blotting: Recommended dilution: 2-4 μg/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli. Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human beta-2-Microglobulin
Specificity	he antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin. Negative Species: Mouse, Bovine, Canine (Dog), Rabbit and Chicken.
Isotype	IgG2a
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Sequential precipitation method with caprylic acid and ammonium sulphate
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	beta-2-microglobulin
Database Link	Entrez Gene 567 Human UniProt ID P61769
Background	Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Synonyms	B2M, Beta-2 microglobulin, Beta 2 microglobulin
Reference Data

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