Neurofilament (NEFM) Mouse Monoclonal Antibody [Clone ID: RNF403]

CAT#: BM6011P

Neurofilament (NEFM) mouse monoclonal antibody, clone RNF403, Purified



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CNY 5,186.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name RNF403
Applications IF, IHC, WB
Recommend Dilution Immunoblotting.
Immunocytochemistry/Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin-Embedded Tissues.
Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and for 1/100-1/500 for Immunoblotting.
Reactivity Hamster, Human, Monkey, Rat, Xenopus
Host Mouse
Clonality Monoclonal
Immunogen Neurofilament preparation of calf brain tissue.
Specificity RNF403 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Gene Name neurofilament, medium polypeptide
Background Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Synonyms Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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