DNA Mouse Monoclonal Antibody [Clone ID: AC-30-10]

CAT#: BM5520P

DNA mouse monoclonal antibody, clone AC-30-10, Purified



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CNY 5,115.00


货期*
5周

规格
    • 100 ug

Cited in 1 publication.

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Specifications

Product Data
Clone Name AC-30-10
Applications IF, IHC
Recommend Dilution Cell Culture.
Dot Blot on nitrocellulose membrane (after baking at 70°C).
Immunohistochemistry on Frozen and Paraffin Embedded Tissue.
Working DilutionAfter reconstitution with 1 ml distilled water, dilute further 1/10 with PBS, pH 7.4 for Immunohistochemistry.
Incubation Time1h at RT.
Reactivity All Species
Host Mouse
Clonality Monoclonal
Immunogen Double- and single-stranded DNA
Specificity Antibody clone AC-30-10 is a reliable Positive Control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases.
It can also be used for sensitive detection of mycoplasma contamination in cell cultures.
Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA.
The antibody recognizes DNA in all DNA-containing species.
Tested Reactivities on Cultured Cell Lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.
Formulation PBS, pH 7.4
State: Purified
State: Lyophilized purified IgM fraction
Stabilizer: 0.5% BSA
Preservative: 0.09% Sodium Azide
Reconstitution Method Restore in 1 ml distilled water
Purification Gel Filtration
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Background dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Synonyms Deoxyribonucleic Acid
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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