IL1RA (IL1RN) Mouse Monoclonal Antibody [Clone ID: 1384.92]

Specifications

Product Data
Clone Name	1384.92
Applications	ELISA
Recommend Dilution	Suitable for ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human IL-1Ra.
Specificity	Monoclonal antibody 1384 is useful for studying biological effects of IL-1Ra in vitro. Reacts with IL-1 receptor antagonist. Negative with Human IL-1 beta, IL-1 alpha. Negative with Mouse IL-1Ra. Antigen Distribution: The antigen is produced and secreted by monocytes and macrophages after stimulation with pro-inflammatory cytokines, LPS, and in vitro by immunoglobulins coated to surfaces after an induction phase of 4h. In vitro the quantity of IL-1Ra secreted depends on the dose and type of stimulus.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS), pH 7.2, no stabilizer and 0.05% v/v Kathon (TM) CG as a presertative. State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.2 ml distilled water (stock solution).
Concentration	1 mg/ml
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	interleukin 1 receptor antagonist
Database Link	Entrez Gene 3557 Human UniProt ID P18510
Background	The interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring molecule that shares homology with IL-1α and IL-1β. It binds competitively to IL-1 receptors Type I and Type II with the same affinity as does IL-1, but does not induce any intracellular response. Two structural variants of IL-1Ra have been described: a 17 kDa form that is secreted from monocytes, macrophages, neutrophils, and other cells (sIL-1Ra) and an 18-kDa form that remains in the cytoplasm of keratinocytes and other epithelial cells, monocytes, and fibroblasts (icIL-1Ra). An additional 16-kDa intracellular isoform of IL-1Ra has recently been described in neutrophils, monocytes, and hepatic cells. Both of the major isoforms of IL-1Ra are transcribed from the same gene through the use of alternative first exons. The production of IL-1Ra is stimulated by many substances including adherent IgG, other cytokines, and bacterial or viral components. The tissue distribution of IL-1Ra in mice indicates that sIL-1Ra is found predominantely in peripheral blood cells, lungs, spleen, and liver, while icIL-1Ra is found in large amounts in skin. Studies in transgenic and knockout mice indicate that IL-1Ra is important in host defense against endotoxin-induced injury. IL-1Ra is produced by hepatic cells with the characteristics of an acute phase protein. Endogenous IL-1Ra is produced in numerous experimental animal models of diesease as well as in human autoimmune and chronic inflammatory diseases. The use of neutralizing anti-IL-1Ra antibodies has demonstrated that endogenous IL-1Ra is an important natural antiinflammatory protein in arthritis, colitis, and granulomatous pulmonary disease.
Synonyms	IL1F3, IL-1ra, IL-1RN, IRAP, IL1 inhibitor, ICIL-1RA
Reference Data

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