MHC Class I H-2 Dd, H-2(k,q,s) Rat Monoclonal Antibody [Clone ID: ER-MP42]

Specifications

Product Data
Clone Name	ER-MP42
Applications	FC, IHC
Recommend Dilution	Immunohistology on frozen sections (1:200; Acetone, Formaldehyde, Glutaraldehyde are suitable fixatives). Does not react on routinely processed paraffin sections. FACS (1:50 - 1:100).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse macrophage precursor cells
Specificity	Monoclonal antibody ER-MP42 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd, H-2k,q,s. A weaker reactivity is found in mouse strains with the following haplotypes: H-2p,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by ER-MP42.
Isotype	IgG2a
Formulation	PBS, pH 7.2, 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative State: Purified State: Lyophilized affinity purified IgG fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml (after reconstitution)
Conjugation	Unconjugated
Note	MHC class I antigens are heterodimers consisting of one α chain (44kDa) with β2-microglobulin (11.5 kDa). The epitope recognized by ER-MP42 is resistant to 0.05% glutaraldehyde, 1% paraformaldehyde and acetone.
Reference Data

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