E Cadherin (CDH1) Mouse Monoclonal Antibody [Clone ID: 67A4.2.1]

Specifications

Product Data
Clone Name	67A4.2.1
Applications	FC, IHC
Recommend Dilution	Suitable for Immunohistochemistry (frozen sections: 4-8 µg/ml, 1/50-1:100) and FACS. Suggested positive control: Human tonsil. Does not react on routinely processed paraffin sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Breast carcinoma cell line T47D
Isotype	IgG1
Formulation	PBS pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.09% sodium azide as preservative. State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water. For best results, dilute in TBS or other phosphate-free buffer containing 10 mM Ca2+.
Concentration	0.4 mg/ml
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cadherin 1
Database Link	Entrez Gene 999 Human UniProt ID P12830
Background	E-Cadherin, also called L-CAM (Liver Cell Adhesion Molecule) is an epithelial cell adhesion molecule and plays an important role in the formation of cell-cell contacts in epithelia irrespective of their origin as ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex and is as such important in maintaining epithelial integrity. Over 90% of malignancies are carcinomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-cadherin expression. Therefore, the expression of E-cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.
Synonyms	Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum
Reference Data

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