alpha 1 Antichymotrypsin (SERPINA3) Mouse Monoclonal Antibody [Clone ID: ACT14C7]

Specifications

Product Data
Clone Name	ACT14C7
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.16 µg/ml (1/2500). Immunohistochemistry on Paraffin Sections: 0.16 µg/ml (1/2500), Microwave pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes alpha-1 Antichymotrypsin in paraffin embedded and frozen Human cells and tissues. Monocytes and macrophages are recognized in tonsils.
Isotype	IgG1
Formulation	PBS buffer pH 7.2 containing 0.1% Kathon as preservative and 5 mg/ml BSA as stabilizer State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	serpin family A member 3
Database Link	Entrez Gene 12 Human UniProt ID P01011
Background	The protein is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variation in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Synonyms	SERPINA3, AACT, GIG24, GIG25
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7min. at 700Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum
Reference Data

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