Interdigitating cells (access. T-cell Macrophages) Mouse Monoclonal Antibody [Clone ID: X-12]

Specifications

Product Data
Clone Name	X-12
Applications	FC, IHC
Recommend Dilution	Suitable for Immunohistochemistry on frozen sections (0.25 µg/ml, 1/800) and FACS. Suggested positive control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human mononuclear peripheral blood cells.
Specificity	BMA X-12 is useful for the detection of a subpopulation of human interdigitating (reticulum) cells in T-cell areas of lymph nodes and spleen and their in vitro correlates. It is also useful for the detection of a macrophage subpopulation in the thymus. Detects human Interdigitating (reticulum) cells and a subpopulation of tissue macrophages. Antigen distribution on isolated cells: The antigen is found on a subpopulation (approximately 5%) of circulating monocytes and peritoneal macrophages, as well as on in vitro LPS and IFN stimulated macrophages and on all macrophages after 3 weeks in culture. It is absent from all other blood cells. Tissue sections: The antigen is found on interdigitating cells in the T-cell dependennt areas of lymph nodes and spleen. In the thymus, it is also found on a subpopulation of macrophages in trabeculae, cortex and medulla. It is absent in the skin. A minor but distinct population of leukocytes in the kidney cortex is also positively stained by X-12. Cross reactivity: Negative in rat and mouse.
Isotype	IgG1
Formulation	PBS pH 7.2 containing 0.01% thiomerosal as preservative and 10 mg/ml BSA as stabilizer. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum
Reference Data

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