Integrin alpha 5 (ITGA5) Mouse Monoclonal Antibody [Clone ID: X-6]

Specifications

Product Data
Clone Name	X-6
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: use 10 µl stock solution to label 10e6 cells. Immunohistochemistry on Frozen Sections: 4 µg/ml (1/100). Suggested Positive Control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Monocytes.
Specificity	This antibody X-6 is useful for the detection of the Fibronectin Receptor, or CD49e. Detects Human granulocytes, monocytes and platelets. Cross reactivity: unknown. X-6 detects an antigen of 154-180kD by Immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes; positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95% cord blood CD34+ cells, weak reaction on resting neutrophils. Staining increases with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes positive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to a decrease in the number of positive cells. Positive with cell lines U937, DH39 & MEP, HL-60, NB4, HMC-1. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.
Formulation	Stock solution contains PBS, pH 7.2 with 0.01% Thiomerosal as preservative and 10 mg/ml BSA as stabilizer. Label: Biotin State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water
Concentration	0.4 mg/ml
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 5
Database Link	Entrez Gene 3678 Human UniProt ID P08648
Background	The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.
Synonyms	Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA
Reference Data

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