MRP8 (S100A8) Mouse Monoclonal Antibody [Clone ID: S13.67]

Specifications

Product Data
Clone Name	S13.67
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA. Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200). Immunohistochemistry on Paraffin Sections: 5-10 µg/ml (1/20-1/50). No pretreatment for antigen retrieval necessary. Suggested Positive Control: Human tonsil. Has been described to work in FACS and Dot Blots.
Reactivity	Human, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Cultured Human Monocytes.
Specificity	S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. S 13.67 is useful for the identification of the 8kD S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope). Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively. Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).
Isotype	IgG1
Formulation	PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% (v/v) Kathon CG as a preservative. Label: Biotin State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 0.5 ml distilled water to make stock solution.
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store the loyphilized antibody at 2-8°C. Aliquot and store reconstituted antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	S100 calcium binding protein A8
Database Link	Entrez Gene 6279 Human UniProt ID P05109
Background	MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.
Synonyms	S100-A8, CAGA, MRP-8, CFAG
Reference Data

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