BLM pThr99 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1:500~1:1000. Immunohistochemistry on paraffin sections: 1:50~1:100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome (KLH-conjugated)
Specificity	The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography using epitope-specific peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	159 kDa
Gene Name	Bloom syndrome RecQ like helicase
Database Link	Entrez Gene 641 Human UniProt ID P54132
Background	Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Synonyms	Bloom syndrome protein, RecQ protein-like 3, DNA helicase, RecQ-like type 2, RECQ2, RECQL3
Reference Data

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