Sprouty 4 (SPRY4) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1/500-1/2000.  Immunohistochemistry: 1/100-1/500.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from N-terminal region of Sprouty homolog 4 (Spry-4)
Specificity	Reacts with Human 32 kDa Spry-4 protein. Cross reacts with Mouse, Bovine and Chicken.
Formulation	0.1M Tris, 0.1M Glycine and 2% Sucrose State: Purified State: Lyophilized purified antibody Preservative: None
Reconstitution Method	Restore in distilled water.
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store the antibody at -20°C. Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	sprouty RTK signaling antagonist 4
Database Link	Entrez Gene 81848 Human UniProt ID Q9C004
Background	Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
Synonyms	Spry-4, Sprouty 4
Reference Data

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