mGluR1a / GRM1a Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at 1/2000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Isotype	IgY
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified IgY fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. This product is photosensitive and should be protected from light.
Gene Name	glutamate metabotropic receptor 1
Database Link	Entrez Gene 2911 Human UniProt ID Q13255
Synonyms	Metabotropic glutamate receptor 1, Gprc1a, Mglur1
Reference Data

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