LC3 pSer12 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1/1000. Dot Blot: 1/500.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic phosphopeptide between 1-30 amino acids surrounding Ser12 of Human LC3 (APG8a).
Specificity	Recognizes Phospho LC3C- Serine12
Formulation	PBS State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) Sodium Azide
Concentration	lot specific
Purification	Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	14272 Da
Gene Name	microtubule associated protein 1 light chain 3 alpha
Database Link	NP_115903.1 Entrez Gene 84557 Human UniProt ID Q9H492
Background	MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Synonyms	MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1 light chain 3-like protein 1, MAP1LC3A, LC3A
Reference Data

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