Penk Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western Blot: Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Frozen Sections: 0.02-0.05 μg/ml. Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus.
Reactivity	Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Enkephalin antibody was raised against a synthetic peptide of Enkephalin.
Specificity	This antibody recognizes Proenkephalin (PENK).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	preproenkephalin
Database Link	Entrez Gene 18619 Mouse UniProt ID P22005
Synonyms	preproenkephalin; proenkephalin
Reference Data

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