Cytochrome b reductase 1 (CYBRD1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Peptide ELISA: 1/16000 (Detection Limit). Western Blot: Experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 31.6kDa according to NP_079119. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry on Paraffin Sections: 2-5 µg/ml. In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. An anonymous customer found positive results on Mouse duodenum.
Reactivity	Bovine, Canine, Human, Porcine
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-Terminus of Human CYBRD1 (NP_079119.3)
Specificity	This antibody is expected to recognise isoform 1 of CYBRD1 (NP_079119.3) only.
Formulation	Tris saline, pH~7.3, 0.02% Sodium Azide State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cytochrome b reductase 1
Database Link	Entrez Gene 79901 Human UniProt ID Q53TN4
Synonyms	Cytochrome b reductase 1, DCYTB, FRRS3, Duodenal cytochrome b, Ferric-chelate reductase 3
Reference Data

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